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KMID : 0620920010330040205
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Experimental & Molecular Medicine
2001 Volume.33 No. 4 p.205 ~ p.208
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Study of ethidium bromide interaction peculiarities with DNA
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P O Vardevanyan/P O Vardevanyan
A P Antonyan/G A Manukyan/A T Karapetyan
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Abstract
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The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M ≤ muNa+ ≤ 2.0 x 10(-2) M has been investigated. It has been revealed that at the certain high ligand-DNA ratios (r(b)) the transition interval of the complex-(ΔT) becomes equal to that of DNA itself (ΔTo). It has been shown that the values of r(b) at which δ ΔT=ΔT-ΔT0=0 depends on ionic strength of a solution. Further increasing of ligand concentration leads to its conversion from stabilizer into the destabilizer of the double-stranded DNA.
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KEYWORD
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DNA, ethidium bromide, mode of binding, bell-shape curve, melting curve,
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